Department of Orthopaedic Surgery

Thomas P. Lozito, PhD

  • Research Assistant Professor

Education & Training

  • PhD, University of Cambridge
  • BS, Johns Hopkins University

Representative Publications

1. Lozito TP, Tuan RS. Lizard Tail Regeneration Combines Aspects of Fracture Healing and Blastema-Based Regeneration. Development. 2015 Under Review.
2. Lozito TP, Tuan RS. Lizard Tail Regeneration: Regulation of Two Distinct Cartilage Regions by Indian Hedgehog. Dev Biol. 2015 Mar 15;399(2):249-62.
3. Lozito TP*, Lin H*, Alexander PG*, Gottardi R, Tuan RS (*Equal Author Contribution).Stem cell-based microphysiological osteochondral system to model tissue response to interleukin-1β. Mol. Pharmaceutics. 2014 Jul 7; 11(7):2203-12.
4. Lozito TP, Tuan RS. Endothelial and cancer cells interact with mesenchymal stem cells via both microparticles and secreted factors. J. Cell Mol Med. 2014 Dec;18(12):2372-84.
5. Lozito TP, Jackson WM, Nesti LJ, Tuan RS. Human mesenchymal stem cells generate a distinct pericellular zone of MMP activities via binding of MMPs and secretion of high levels of TIMPs. Matrix Biology. 2014 Feb;34:132-43.
6. Boyette LB, Creasey OA, Guzik L, Lozito TP, Tuan RS. Human bone marrow-derived mesenchymal stem cells display enhanced clonogenicity but impaired differentiation with hypoxic preconditioning. Stem Cells Transl Med. 2014 Feb;3(2):241-54.
7. Moon HJ, Yurube T, Lozito TP, Pohl P, Hartman RA, Sowa GA, Kang JD, Vo N. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage. 2014 Feb;22(2):344-54.
8. Alexander PG, Gottardi R, Lin H, Lozito TP. Three-dimensional osteogenic and chondrogenic systems to model osteochondral physiology and degenerative joint diseases. Exp Biol Med. 2014 Sep;239(9):1085-95.
9. Lozito TP, Alexander PG, Lin H, Gottardi R, Cheng AW, Tuan RS. 2013. 3-D Osteochondral Microtissue to Model Pathogenesis of Osteoarthritis. Stem Cell ResTher. 2013;4 Suppl 1:S6.
10. Cui C, Chatterjee B, Lozito TP, Zhang Z, Francis R.J, Yagi H, Swanhart LM, Sanker S,Francis D, Yu Q, San Agustin JT, Puligilla C, Chatterjee T, Tansey T, Liu X, Kelley MW, Spiliotis ET, Kwiatkowski AV, Tuan RS, Pazour GJ, Hukriede NA, Lo CW. Wdpcp, a PCP Protein Required for Ciliogenesis, Regulates Directional Cell Migration and Cell Polarity by Direct Modulation of the Actin Cytoskeleton. Plos Biology. 2013 Nov;11(11).
11. Lozito TP, Tuan RS. Endothelial cell microparticles act as centers of MMP-2activation and for vascular matrix remodeling. J Cell Physiol. 2012 Feb;227(2):534-49.
12. Jackson WM, Lozito TP, Djouad F, Kuhn NZ, Nesti LJ, Tuan RS. 2011. Differentiation and regeneration potential of mesenchymal progenitor cells derived from traumatized muscle tissue. J Cell Mol Med. 2011 Nov;15(11):2377-88.
13. Lozito TP, Tuan RS. Mesenchymal stem cells inhibit both endogenous and exogenous MMPs via secreted TIMPs. J Cell Physiol. 2011 Feb;226(2):385-96.
14. Lozito TP, Kuo CK, Taboas JM, Tuan RS. Human mesenchymal stem cells express vascular cell phenotypes upon interactions with endothelial cell matrix. J CellBiochem. 2009 Jul 1;107(4):714-22.
15. Lozito TP, Taboas JM, Kuo CK, Tuan RS. Mesenchymal stem cell modification of endothelial cell matrix regulates their vascular differentiation. J Cell Biochem. 2009Jul 1;107(4):706-13.
16. Husain FT, Lozito TP, Ulloa A, Horwitz B. Investigating the Neural Basis of the Auditory Continuity Illusion.  J. Cogn. Neurosci. 2005 Aug;17(8):1275-92.

Research Interests

 

Lizards and salamanders both exhibit the amazing ability to regenerate amputated tails.However, while the salamander-regenerated tail is a perfect copy of the original, the lizard regenerates an “imperfect replicate”. The most striking of these “imperfections” concerns the skeleton, which takes the form of an unsegmented cartilage tube rather than a vertebral column.My research compares skeletal regeneration in lizards (Lepidodactylus lugubris) and salamanders (Ambystoma mexicanum), looking for what they have in common, how they differ,and why they end with such different regenerative outcomes. For example, we have determined that lizard and salamander blastemas (the collection of cells that differentiate into the tissue ofthe regenerated tail) form in very different ways, findings with potential for improving mammalian regeneration.

Research Grants

“Why don’t lizards regenerate perfect tails like salamanders?”
4/1/2016 – 4/1/2020
NIH / NIGMS
R01